Bioconversion of 2-amino acids to 2-hydroxy acids by Clostridium butyricum

Deamination of amino acids by Clostridium botulinum.

The types of Clostridium botulinum were found to differ in their abilities to deaminate single amino acids. Cells were prepared from 24to 36-hr cultures grown at 37 C in screw-cap tubes in a medium containing (grams per liter); Trypticase (BBL), 15; Phytone (BBL), 5; yeast extract, 5; K2HP04, 8.5; KH2PO4, 1.5; NaCl, 1; MgSO4. 7H20, 0.25; FeSO4.7H20, 0.0125; and sodium thioglycolate, 1. The cell...

Performance of 2-Amino Tetraphenyl Porphyrin as Stationary Phase in RP-HPLC of Amino Acids

The search for new stationary phases has been one of the predominant concerns in high performance liquid chromatography (HPLC) in order to achieve better resolutions, longer column lives, and reduce the time of analysis. A chromatographic packing for separation of underivatized amino acids (AAs) were prepared by dynamically coating 2-amino tetraphenyl prophyrin (atpp) on a C-18 reversed-pha...

Biosynthesis of amino acids in Clostridium pasteurianum.

1. Clostridium pasteurianum was grown on a synthetic medium with the following carbon sources: (a) (14)C-labelled glucose, alone or with unlabelled aspartate or glutamate, or (b) unlabelled glucose plus (14)C-labelled aspartate, glutamate, threonine, serine or glycine. The incorporation of (14)C into the amino acids of the cell protein was examined. 2. In both series of experiments carbon from ...

Acetylation of amino acids by enzymes of Clostridium kluyveri.

The experiments were done with dried cells of C. kluyveri, Lots Q and R, prepared by the method of Stadtman and Barker (2). Lot R contained 1.2 pM of volatile acids, consisting mostly of caproic acid with a little acetic and butyric acids, and 3.2 I.LM of non-protein amino nitrogen per 25 mg. of dried cells. The enzymes responsible for acetylation of amino acids are pot readily inactivated by e...

Occurrence of 2-hydroxy Fatty Acids in Animal Tissues.